HeLa. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. 046 to 0. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. 7°C and the bottom to 36. Dual injectors for kinetic assays. Compare Tool. All readings were taken in clear, polystyrene 96-shallow-well, flat-bottom plates. com Molecular Devices (Germany) GmbH ismarcring ierach an der iss erman ©2019 Molecular Devices, LLC. Well scanning With well scanning, multiple points within each well can be read, providing a high. Resipher 32x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 32x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 32 wells Utilizes Lucid Lab Cloud software for connection to any…. White light and fluorescence images of the 6-well plates were captured with the ClonePix 2 System. Users can choose to process all plates within the magazine or select a specific number. Luminescence applications and assays. Compare this item. Yes—whole plate dispenser (96 or 384 well) Hamamatsu: FDSS7000EX: High throughput screening system: Yes (optional feature). For more sample throughput, the system can be easily integrated into a full robotic The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. Based on this customer experience and feedback, the Infinite 200 PRO microplate plate reader has been continuously engineered for. A. 0, or. Features of Clear 96-Well Microplates: • Clear plates for colorimetric ELISA and other assays. Microplate readers from Tecan; cutting edge bench-top microplate readers lead the industry in both versatility and performance. 2 pM 384–well: 3 pM 96–well: 2 pM 384–well: 4 pM 1 0200-7012POS Glow for 96-, 384-, and 1536-well plates Here, we demonstrate that with Molecular Devices SpectraMax® microplate readers and the Quant-iT PicoGreen assay, users can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA. In labs where throughput is a top priority, a device that can screen 96- to 1536-well microplates in under one minute is worthwhile. The device was able to achieve 3σ limits of detection of ca. 0200-6262. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. The equation that allows one to calculate absorbance from % transmittance is. Max, FlexStation, Gemini, and earlier instruments as well as import and analysis of data files from FLIPR and Analyst instruments. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). 2 OD at 200 nm; this decreased to 0. After incubation of the cells with compounds, add 100 µL (for 96-well plates) or 25 µL (for 384-well plates) of Dye Solution per test well. Compact design Minimum volume and maximum throughput with 96- or 384-well microplate compatibility Powerful data. Published Dec 16, 2020 | Updated Dec 16, 2020. UV absorbance detection. ₹ 1,80,000 Get Latest Price. Bioz Stars score: 86/100, based on 40 PubMed citations. guaranteed 25 fmol/200 µL fluorescein (125 pM), black 96-well plate; 1 second integration time 15 fmol/75 µL. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. SoftMax Pro Software minimizes setup time for detection and automates standard curve fitting and sample quantitation. We would like to show you a description here but the site won’t allow us. 6-384 (96- and 384-well with pipetting) 96-well microplates. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. Click Add to create the plate. LogPhase 600 is controlled with a software App that provides a simple, yet powerful, user experience. 0 fmol/well FITC 200 µL in 96 wells Bottom-read detection limit. 0 . Mix well by trituration or plate shaker and incubate for 2 to 5 minutes at room temperature, protected from light. 96-, 384-, and 1536-well microplates. 500 USD. 1% in white 96- and < 0. The BioTek Synergy H1 multimode microplate reader is modular and upgradable to include variable-bandwidth monochromator optics for fluorescence excitation and emission wavelength selection, plus luminescence and UV-Vis absorbance. 00 Add to cart; Molecular Devices EMax Microplate Reader $. 312 gain calibration failed. These are the default kind of cuvette & 96 well plate stocked by the lidstrom lab as of 11/2013. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. 96 wells 384 wells 1 pM 1 pM Optimized bottom sensitivity (fluorescein) 96 wells 384 wells 2 pM 2. style. Plate chamber design of the SpectraMax category 1 microplate readers. Plate types Flat and round 96-well plates Data output Export to PC Detection system Single channel silicon photodiode Reading speed 25 seconds Software SoftMax Pro Software Shaking None Temperature control None Linearity 0. com. Applications include ELISA, protein quantification, enzyme kinetics and cell-based assays. Versa Max. The SpectraMax i3x Multi-Mode Microplate Reader has been validated for use with the ValitaTiter and ValitaTiter Plus assays. 20 µL (96-well plates) or 10 µL (384. Selects either 96-well or 384-well plate for a stand-alone read. This robust system is capable of reading 96 wells, each with its own sample beam and reference detector. Supplier: Molecular Devices Next generation SpectraMax® absorbance microplate readers. T = I/I 0 and %T = 100 (T). g. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. Molecular Devices delivers a robust screening platform to address real-time kinetic cell-based assays with the FLIPR Tetra® System. *For Corning UV plates, mean values at 260 nm ranged from 0. The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. 96- and 384-well. This is equivalent to having 511 built-in filters,The instruments offer 96- and 384-well microplate-to-microplate fluid transfer (8 or 16 wells at a time), and can read microplates having 6, 12, 24, 48, 96, and 384-well formats. Categories: Plate Readers. More Product Information. 0 . 6- to 1536-well plates 4 96- to 384-well plates 4 With up to five modes of detection, F3/F5 Multi-Mode Microplate Readers support a wide variety of applications in life science research. Step 3: Wash microplate – Unbound material is washed away, leaving only the antigen of interest. perfect for kinetic readings involving growth curves at 5 seconds per plate. When samples are added to the wells, the Protein G molecules are resuspended and binding occurs. $14,995. Place the microplate in the drawer. 0% ± 0. 00. Quantitative, whole well cell confluence for every well. Molecular Probes 96-well microplates for fluorescence-based assays are black-walled, clear-bottom 96-well microplates that minimize well-to-well crosstalk and. SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. the absorbance at 340 nm at 37 °C was measured every 30 s using SpectraMax M5 Microplate. With SoftMax Pro Software, the PathCheck Sensor automatically normalizes the well absorbance. Reconstitution of. 200, the Bio-Tek µQuant™, the Bio-Rad UltraMark™, the Molecular Devices SPECTRAmax® 190 and 250 plate readers. White 96-well platePlate formats. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. Uncontrolled Copy for Training and Reference Purposes Only . The SpectraMax L reader is easily integrated with the optional StakMax microplate stacker from Molecular Devices for automated multiplate assays. 1. Fluorescence Microplate Reader Comparison Chart. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. Click on the crosshair and move it so that it is centered over the well image. Products : SpectraTest Validation Plates ABS2, SpectraTest Validation Plates ABS1, SpectraTest Validation Plates FL1, SpectraTest Validation Plates LM1. UV cuvettes are ideally suited for measurements at 260 nm, 280 nm and in the visible range. SpectraTest FL1 Fluorescence Validation Plate User Guide Molecular DevicesData are shown as the mean ± s. absorbance microplate reader GEA. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). Whether running fluorescence imaging or brightfield assays, the automated imager features a comprehensive portfolio of preconfigured protocols for cell-based assays to shorten the learning curve, so. 0025 OD from 0. SpectraDrop High-Throughput Screening (HTS) Kit. 5 ng per well. Absorbance Plate Reader Comparison Chart. We would like to show you a description here but the site won’t allow us. Explore the best practices for running absorbance-based assays how Molecular Devices absorbance readers can be utilized to optimize said absorbance-based assays. Cell line development teams will realize savings on time and resources, as well as faster time-to-market by utilizing a single technology for measuring a critical quality attribute duringOpening the Drawer Manually on page The SpectraMax ABS can accommodate standard 96-well plates. EMax. Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. 9000-0756. 0200-6263. We would like to show you a description here but the site won’t allow us. 5 pM Luminescence performance Wavelength range 300–850 nm Wavelength selection Choice of simultaneous detection of all wavelengths or selection in 1. Never order or replace filters to optimize an assay again!6-, 12-, 24-, 48-, 96- and 384-well plates: Tablet PC: Windows® 8. These instruments deliver high precision and speed when reading 96 Well Microplates for single endpoint measurements and kinetic assay data gathered over time. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384. 00. Tristar 3 Multimode Microplate Reader. Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. It is designed to work seamlessly with a variety of 96 well microplate readers, including popular brands such as Agilent™, Berthold™, Biotek™, BMG LABTECH™, Molecular Devices™, Perkin Elmer™, Promega™, Tecan™, and. #W50-100) SpectraMax® ABS Plus Microplate Reader (Molecular Devices cat. These include real-time PCR, measuring yields of RNA preparations for microarray experiments, reverse transcription PCR (RT-PCR), differential display PCR,. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. FlexIII. x. Incubation. The procedures in this protocol support the 96-well reaction plate. 4 (Molecular Devices cat. Detect dim samples with unsurpassed sensitivity; Avoid saturation issues with nine orders of dynamic range. Ensure that cells reach around 80–90% of confluence on the day of the. x. A Costar 24-well tissue culture plate with col1 = blanks and doubling cell a list of plates specifically supported shows, 96, 384, 24, 12, 6 well types. Absorbance values were read on the SpectraMax iD5 reader using a preconfigured BCA assay protocol (the SpectraMax iD3 reader is also suitable for these assays, as it has the same absorbance performance as the SpectraMax iD5 reader). Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. F3/F5 Multi-Mode Microplate ReadersAnalyst® Multimode Reader—one of the following: • Acquest or Analyst AD or HT with at least v. Temperature control: 4°C above ambient to 45°C. It offers extremely low absorbance background, excellent. To maintain confidence in the standards, Molecular Devices recommends having validation plates recertified at one-to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, andthe iD5 reader’s filter system. #E010) Pyroplate 96-well microplate (ACC cat. Endpoint, kinetic, spectrum. 1. Published Dec 16, 2020 | Updated Dec 16, 2020. 003 fmol 0. Versa Max. 2b,c. Used. • High antibody-binding surface improves the overall performance in. Specifications subject to change without notice. This page contains the. VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. 100 μL of working reagent was added to each well after the 10-minute preincubation step. 4 decades . Self-righting base, up to 40 degrees past center without dispenser. What is the dead volume in 96 and 384 well compound plates? Dead volume is 5 micro liters. Sample by eye within . For the name of the nearest authorized Molecular Devices Corporation service facility please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. The SpectraMax Paradigm Platform was able to quantify the protein down to the low picomolar level using an AlphaLISA method (Figure 1). However, for greater flexibility, some instruments can accommodate 6- to 1536-well plate formats. In addition, users can define individual reagents and concentrations to be delivered to each well. (96- or 384-well) simultaneously and with high temporal resolution. 5039228D 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 11 ComputerIntegration 12 Applications 12 Optics 13 PlateControls 13The microplate reader has been around for more than three decades and continues to serve as a must-have workhorse of clinical, academic research and industrial laboratories alike. Absorbance equation. The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. Four wash/rinse bottles are included and configurable for both 96- and 384. #6590-0137) Optimal instrument settings for ValitaTiter (P/N VAL003 or VAL013, left table) and ValitaTiter Plus (P/N VAL004 or VAL014, right table) assays on the SpectraMax iD5 reader are shown below. Dark blue indicates well will be read. Use standard plate unless you see your type listed. SoftMax Pro GxP Software version 5. Unparalleled performance on a personalized platform. Browse for different fluorescent readers below and compare across different instrument suppliers. #R8195. When a. It features a modular design that allows users to add or change detection cartridges according to their needs. Standard features on the EMax® Plus Microplate Reader include the ability to measure both flat and round 96-well microplates, automatic lamp calibration prior to each reading (ensuring automatic measurements), powerful curve fitting protocols and statistical analysis tools, and integrated instrument control and data analysis with SoftMax® Pro Software. Plate Shaking. Molecular Devices SpectraMax Plus Microplate Reader Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader Sale Price: $6,495. Conventional technique: subjective, time-consuming. In addition, the iD5 reader measures TRF and FP and. #3693; n = 4 for each standard). Two days before the experiments, cells are seeded into the clear-bottom 96-well plates (approximately 10,000 cells per well for cancer cell lines and 30,000–40,000 cells per well for fibroblasts and NPC; see Note 5) and cultured in the media suited for users’ cell lines. This can increase throughput and efficiency, thus saving time and space in the lab. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices. The Versamax Microplate Reader is built around a grating monochromator. x. Software Validation. Polypropylene Deep well Plates are used as small test tubes because of easier sealing, flat or conical shape, square and round well formats with lids and cap. 012 fmol 0. Gemini XPS & EM readers measure fluorescence on a variety of sample formats from 6- to 384-well microplates. # SMP54-GXP- 10UL) Pyrochrome Assay chromogenic test reagent (ACC cat. Click Create. Molecular Devices SpectraMax i3 Multi-Mode Microplate Reader Pred i3x - AV. The SpectraMax® L microplate reader measures flash and glow luminescence assays in both 96- and 384-well plates. Recertification service. These robust readers have been placed in labs from. Temperature control (RT+3°C – 42°C) Liquid dispensers with reagent heater and stirrer. 340 PC384. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. tools to expand the boundaries of their research. Detection of KASP genotyping reactions. Primary considerations when choosing a microplate reader are throughput and flexibility. 00 USD. 5 mm left a residual volume of 100 µL in each well of a 96-well plate or 25 µL for a 384-well plate. A microplate adapter is required in the reading chamber drawer for 96- and 384-well assay plates. A = Log 10 (I 0 /I). From the dropdown Menu, select Wellplates. SoftMax Pro GxP Software version 5. Upon completion of a plate read, data are delivered to your computer in an Excel-compatible format. Read the plate on the SpectraMax M5, M5e, or FlexStation 3 reader using the optimized settings shown above. These models can no longer be repaired. ©2022 Molecular Devices - All rights. x. M2. A Costar® 24-well tissue culture plate with col1 = blanks and doubling cell. Both reactions can be easily measured in Molecular Devices’ new SpectraMax® L microplate luminometer. Please refer to the Obsolete Microplate Reader Models for more information. Janet got excellent results using them and they are only ~$1. We would like to show you a description here but the site won’t allow us. 0% Normal and Rapid modes, 96- and 384-well plates; Absorbance Repeatability: 0. #ABS PLUS) Methods. The LysoTracker fluorescence at ex/em 574/594 was measured on a Spectramax i3x plate reader (Molecular Devices). The SpectraMax ABS readers can be integrated with Molecular Devices StakMax® Microplate Stacker within . Microplate Readers include the M3, M4, M5, and M5e models. Molecular Devices' microplate readers. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. “The ABS Plus reader comes standard with our patented PathCheck Technology which enables consistent results across a 96- or 384-well plate despite volume differences that may. minutes and begin reading microplates with seven mouse clicks. Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. Spectrofluorometer from Molecular Devices® provides a flexible environment. BioTek Instruments Synergy Neo2 Multi-Mode Reader Fluorescence Measuring Equipment. The list is a standard grouping supplied by Molecular Devices and includes some which cannot be used on this instrument; they can be deleted. #i3x) SpectraMax L Microplate Reader (Molecular Devices cat. The Varioskan LUX multimode reader has received HTRF™ certification from Cisbio Bioassays and DLReady™ validation from Promega Corporation. This eliminates the need for standard curves, and 96–well: 3 mP 384–well: 3 mP 1536–well: 6 mP 1 LUMI Glow luminescence detection for different plates 0200-7014POS Glow for 96-well plates 96–well: 1 pM 96–well: 1. Read the microplate • Make sure the purple plate adapter (if required for your plate reader) is in the microplate reader drawer. NanoQuant plate. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. Quartz Plate found in: Thick Quartz Ground and Polished Plates, Solid Quartz 96 Well Clear Plate with Clear Base, Quartz Parallel Plate Probes for DMA 7. A preconfigured protocol with optimized reader settings enabled reading of a 384-well plate in just two minutes. UT-6550 Microplate Reader is a reliable and robust instrument for a wide. Absorbance was then determined at 450 nm by SpectraMax Paradigm microplate reader (Molecular. 5in (46. Here, we demonstrate validation of the SpectraMax ® i3x Multi-Mode Microplate Reader for NanoBRET assays. xThe SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. 25% and 0. White 96-well plate Plate formats. #C1500) Control standard endotoxin (ACC cat. FLIPR System with the 540-590 nm BP Emission FLIPR Filter installed (Molecular Devices Corporation) Membrane Potential Assay Kit (Molecular Devices Corporation, R-8034) Clear, flat-bottom, black- or clear-wall 384- or 96-well plates (see consumables list in Appendix A) Clear, polypropylene compound plates (see consumables list in Appendix A) Flexibility to read a variety of plate formats. Molecular Devices. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. softmax pro software, gemini em reader, molecular devices stakmax microplate stacker, softmax pro data analysis, spectrofluorometer, monochromator microplate reader, cell migration assays, cell. 96-, 384-, and 1536-well microplates. Two different agonists, acetylcholine and carbachol, were tested. Fluorescence Microplate Reader Comparison Chart. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. Service parts for these models are not being produced and are no longer in stock. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Clear plates will give more cross talk between wells. FlexIII. Values are averages from 5 clean plates, each filled with 200 µL water/well. 0% ± 0. Our. 300 µL of Detergent Compatible Bradford reagent was added to each well. Absorbance Linearity: 2. Wells of 96-well plates (NEST) were pre-coated with 100 μg/ml of collagen solution for 1 hour. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. The system can perform readings. VMax . Prior to the reading of each plate, the microplate reader was “blanked” with a plate containing 0. Plate Background OD, which was used for subsequent tests, was measured as follows. Description: Enter plate name (optional)(2) Wild‑type KRAS inhibits the migration and invasion of pancreatic cancer through the Wnt/β‑catenin pathway Molecular medicine reports January 1, 2023 Hu X, Zhang R, Yao J, Mu B, Zhao C. Powerful, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers. • 0. With various on-board features and capabilities, the ThermoMax. 0 Optical system Filters Power requirementAfter reading the ELISA plate on the EMax® Plus Microplate Reader, the R2 value was 0. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. Microplate Capacity. • Gently wash cells once with 200 μl (for 96 well plate) or 400 μl (for a 24 well plate) warm (37°C) 1X DPBS (containing Mg and CaCl 2). 005 OD Normal and Rapid modes, 96- and 384-well plates;. Bowman Dispensers. The top- and bottom-reading Gemini EM Microplate. Well scans from the Gemini EM Reader illustrating serial dilution of CHO cells using the Live Cell assay from Molecular Probes Live/Dead (Calcein AM/Ethidium Homodimer-1) assay kit. The AquaMax® Microplate Washer is a fully self-contained system, configurable for both 96- and 384-well microplates. Next generation SpectraMax® absorbance microplate readers. We would like to show you a description here but the site won’t allow us. Compare this item. Published Dec 16, 2020 | Updated Dec 16, 2020. 300 µL of Detergent Compatible Bradford reagent was added to each well. Microplate readers, SpectraMax®, with SpectraDrop. Exposure of ApNA to FAAH activity results in the release of the yellow colorimetric dye p-nitroaniline (ε = 13,500 at 382 nm). SpectraMax i3x Microplate Reader, Molecular Devices (Cat# i3X) 96-well white walled clear bottom plates, Costar (Cat# 3903) Purified luciferase, Promega (Cat# E1701). 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. Selects either 96-well or 384-well plate for a stand-alone read. robotics-automation-compatible. Choose the desired plate type, using the upper dropdown menu to choose plate format (96 or 384 wells) and the "Select Specific" menu to choose the specific plate type. The SpectraMax ABS Plus, can accommodate standard 96-well plates and 384-well plates. In the latter case, 8 wells in each column are perfused in series and the 12 columns are. The Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. Published Dec 16,. * Note: Programming the washer with a final aspirate height of 4. Industry standard, 96-well plates. D SpectraMax® M2 SpectraMax® M2e Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part #0112-0102 Rev. Published Dec 16, 2020 | Updated Dec 06, 2021. Determine concentration using the Beer-Lambert LawPage 1 ™ VERSAmax Tunable Microplate Reader Operator’s Manual for Instruments with Serial Numbers Beginning with BN Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0084 Rev. 039. 20 plates/hour (2-minute read time) 96-well 1,920 data points/hour 384-well 7,680 data points/hour 1536-well 30,720 data points/hourMicroplate Reader, For Lab. Part Number. Microplate readers read biological, chemical or physical events that occur on a microtiter plate. No accessories are needed and SoftMax® Pro Software. The clear bottom plates offer the advantage of being able to examine the cells by microscopy during the course of the. B. 00. Plate Readers, Fluorometers & Luminometers; Lab Automation; Molecular Diagnostics. 96-, 384-, and 1536-well microplates. Select up to 3 products. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories. A 96-well microplate reader for absorption spectroscopy was designed, constructed, and tested at a total cost of ca. The approximate dead volumes are: 96-well plates: V-bottom: 20-30 µl U-bottom: 30-40 µl flat-bottom: 50-60 µl 384-well plates: approximately 20 µl. 100 uL of LAL reagent was then added to the wells containing the endotoxin standards, and the kinetic read was started. Making it possible to perform kinetic analysis of solid. The luminescence was measured in a SpectraMax. The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. We would like to show you a description here but the site won’t allow us. #6590-0137) Optimal instrument settings for ValitaTiter (P/N VAL003 or VAL013, left table) and ValitaTiter Plus (P/N VAL004 or VAL014, right table) assays on the SpectraMax iD5 reader are shown below. After incubation of the cells with compounds, add 100 µL (for 96-well plates) or 25 µL (for 384-well plates) of Dye Solution per test well. Plus 384. Therefore you need to ask a physicist. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. Both reactions can be easily measured in Molecular Devices’ new SpectraMax® L microplate luminometer. 96/384-well plate formats. Read the microplate • Make sure the purple plate adapter (if required for your plate reader) is in the microplate reader drawer. The soil was amended with either 1. At 5 seconds to read a 96-well endpoint assay or a 5 second minimum interval between kinetic reads, the VMax Microplate Reader has both speed and performance. SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. Elevated well walls for easier sealing Round bottom formats Square and round well formats Conform to SBS standards Lids and cap mats available Packed in sleeves of 6, 24. 1 – 2. Features. 4ti-0255 96 well assay plate, pyramid flat bottom, clear, non-sterile UV wavelength 4ti-0214 UltraVision Plate 384, black, non -sterile, with UV baseELISA Workflow Using the EMax plus Microplate Reader and MultiWash+ Microplate Washer. Molecular Devices, LLC Plate Formats: 96 or 384 Well Plates; Manifold Channels: Inquire; Dispensing Liquids: Inquire; Citations: Get Quote. When the user applies a set temperature of 37°C, the top is heated to 37. Lucid Scientific. Cayman Chemical. For the 96-well plate, this equates to 1 fg per well to 1 ng per well; for the 384-well plate it is 0. the use of a reader calibrated with primary NIST and NMI standards. 46Click the Read button in the SoftMax Pro 8-channel (96) for FlexStation 3 Reader • 8-channel pipettor • (10) racks of 96-well, FlexStation Pipet Tips (Black) • 96-well yellow plate 0200-6182 Pipettor head kit, 16-channel (384) for FlexStation 3 Reader • 16-channel pipettor • (10) racks of 384-well, FLIPR® Tetra Pipet Tips (Clear) • 384-well yellow plate 0200-6183 Consumables ELISA Plate Readers / Microplate Readers. 1c shows a cover designed to perfuse 8 wells in series on a standard 96-well microplate, as well as a cover for the entire standard 96-well microplate. Each wellThe Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. 01. #C1500) Control standard endotoxin (ACC cat. 1. 315 can't find zero order. Running both standard spectrophotometer and microplate. x. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M4 Microplate Reader transforms each well in a microplate to a fixed. (Molecular Devices cat. The plate was incubated for 10 minutes at room temperature and read at 595 nm on the SpectraMax ABS Plus reader. Microplates, cuvettes (via adapter) Detectors. Plate formats 6, 12, 24, 48, 96, 384 wells Light source Xenon flash lamp (1 joule/flash) Detector Photomultiplier (R-3896). a white 96-well half-area microplate (Corning cat. The color touch screen provides a visual user interface, making programming fast and intuitive. Products : SpectraMax L reader How long does it take to dispense into a 96 well plate with SpectraMax L reader injection? For a 96-well plate it takes a total of 27. They can be read by most microplate readers. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. Cross-talk <0. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase. the Bio-Tek PowerWave™ 200, the Bio-Tek µQuant™, the Bio-Rad UltraMark™, the Molecular Devices SPECTRAmax® 190 and 250 plate readers. $488. x. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Other. One plate received 200 µL of the BCA working reagent, and the other received 200 µL of the Rapid Gold BCA working reagent. #N207) LAL Reagent Water (Lonza cat. Dear Nelly, In a micro plate the fluorescence emission optics are very different from those in a single cell because of obstruction, reabsorption etc. A standard curve consisting of MCF7 cells seeded from 20 to 20,000 per well in a 96-well plate was linear across the entire range with r 2 = 0. A: adipocytes; B: fibroblasts; C: no cells (uptake reagent only). Another consideration, says Amouretti, is plate format. VIS absorbance detection. # SMP54-GXP- 10UL) Pyrochrome Assay chromogenic test reagent (ACC cat. Deep well blocks available in 24, 96 and 384-well formats An affordable, tunable microplate reader for 96-well format Affordable The robot-compatible VersaMax reader is cost-effective—users never have to buy filters. HeLa.